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MASS SPECTROMETRY:
Bruker Ultraflex III MALDI ToF ToF WARP-LC, Denovo protein ID, and imaging software, ClinProt, and Genotools. Finnigan LTQ XL ESI with CID/ETD with a nanosource & HPLC with nano-autosampler, Top-Down, peptide profiling, denovo, and SEQUEST software Finnigan LTQ XL ESI with CID (on order) with a nanosource & HPLC with nano-autosampler, Top-Down, peptide profiling, denovo, and SEQUEST software Finnigan LCQ DECA XP+ with a nanosource & HPLC with nano-autosampler, denovo, and SEQUEST software Applied Biosystems 4000Q Trap with nanosource & HPLC with nano-autosampler & Analyst Software package. Collaborative Mass Spectrometry Labs at UAB (Triple Quads & FTICRs)
OTHER ESSENTIAL EQUIPMENT:
Shimadzu CHIP - High Resolution Chemical Tissue Printer GE LC-MALDI nano collect spotter GE 2D MDLC Nano-HPLC with detector & nano-autosampler Shimadzu Off Line HPLC & Fraction Collector for Top Down Directed Protein ID. Thermo High Capacity Spin Vac with adaptors for common tubes and 96 well plates Sorval floor model cooled centrifuge with common adaptor and for deep 96 well stackable filter extraction plates used for HTP protein extractions of biological fluids Cooled table top centrifuge (96 well plate compatible) Bio-Tek Synergy 2 multiwell plate reader with UV/VIS/FL/L capability Sutter quartz capillary column tip puller with bomb from Next Advance for making nano-columns. Complete cell culture/ Tissue banking facility ( 2 large format -80 freezers, 6ft culture hood, CO2/ O2 incubators, large format 4 degree refrigerator, liquid nitrogen tanks for long term storage, microscope, etc.) Thermo Cryostat for Tissue Imaging by MS and Histology work Station. Complete equipment package to carry out basic synthetic chemistry including organic tags for quantitative MS, and dyes for 1D and 2D page. GE IGPhore System and gel boxes for 2D PAGE technology MJ-Research 96 Well Thermocycler Braun Cell disruptor Perkin Elmer Fluorescence HPLC detector White Light/ Fluorescence box for DNA/RNA/ Protein gels Millipore ultra high purity water system for MS work Other common equipment: including pH meter, programmable heated stirrers, 96 well high speed shaker, vortexers, top loader and analytical balances.
SOFTWARE PACKAGES:
2D PAGE Analysis: Nonlinear SameSpots Peptidomics Discovery: Genedata Expressionist, Refiner MS & Analyst Tissue Imaging: Bruker Daltronics, Flex Imaging LC-MALDI MS: Bruker Daltronics, WARP-LC LCMS Analysis: Trans Proteomics Pipeline (TPP) Tools. All-In-One Proteomics Analysis Tool: Bioinquire, ProteoIQ Data Management System: LabKey/ CPAS Systems Biology Analysis: Cytoscape Statistical Analysis & Graphing: MatLab, Statisica, Origin, SPSS, GraphPad, & ClinProt |
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Finding a Cure Through Discovery Based Sciences |
Equipment & Software |
